|Title||Tautomerism of Uracil Probed via Infrared Spectroscopy of Singly Hydrated Protonated Uracil|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||J.M Bakker, R.K Sinha, T. Besson, M. Brugnara, P. Tosi, J.Y Salpin, P. Maitre|
|Journal||Journal of Physical Chemistry A|
|Type of Article||Article|
|Keywords||AFFINITY, AMINO-ACIDS, COMPLEXES, CYTOSINE, FREE-ELECTRON-LASER, GAS-PHASE, IR, MASS-SPECTROMETRY, MULTIPHOTON DISSOCIATION SPECTROSCOPY, NUCLEIC-ACID BASES, SPECTROSCOPY|
Tautomerism of the nucleobase uracil is characterized in the gas phase through IR photodissociation spectroscopy of singly hydrated protonated uracil created with tandem mass spectrometric methods in a commercially available Fourier transform ion cyclotron resonance mass spectrometer. Protonated Uracil ions generated by electrospray ionization are re-solvated ill a low-pressure collision cell filled with a mixture of water vapor seeded in argon. Their structure is investigated by IR photodissociation spectroscopy in the NH and OH stretching region (2500-3800 cm(-1)) with a tabletop IR laser Source and in the 1000-2000 cm(-1) range with a free-electron laser. In both regions the IR photodissociation spectrum exhibits well-resolved spectral signatures that point to the presence of two different types of structure for monohydrated protonated uracil, which result from the two lowest-energy tautomers of uracil. Ab initio calculations confirm that no water-catalyzed tautomerization occurs during the re-solvation process, indicating that the two protonated forms of uracil directly originate from the electrospray process.
|URL||<Go to ISI>://000261218100014|
|Alternate Title||J. Phys. Chem. A|
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