Cyclic peptide as reference system for b ion structural analysis in the gas phase

TitleCyclic peptide as reference system for b ion structural analysis in the gas phase
Publication TypeJournal Article
Year of Publication2011
AuthorsX. Chen, M. Tirado, J.D Steill, J. Oomens, N.C Polfer
JournalJournal of Mass Spectrometry
Volume46
Issue10
Pagination1011-1015
Date PublishedOct
Type of ArticleArticle
ISBN Number1076-5174
KeywordsB(5) IONS, COLLISION-INDUCED DISSOCIATION, CYCLIZATION, EXCHANGE, FRAGMENT ION, H/D EXCHANGE, hydrogen/deuterium, HYDROGEN/DEUTERIUM EXCHANGE, infrared multiple photon dissociation spectroscopy, INFRARED-SPECTROSCOPY, LEU-ENKEPHALIN, macrocycle, OXAZOLONE, peptide fragmentation mechanism, PROTONATED PEPTIDES, RESONANCE MASS-SPECTROMETRY
Abstract

Infrared multiple photon dissociation spectroscopy and hydrogen/deuterium exchange methods are used to confirm the macrocylic structure of a b(6) peptide fragment by direct comparison with a synthetically made cyclic peptide. The acetylation of the peptide N-terminus results in the inhibition of the macrocyclic formation, supporting the "head-to-tail" cyclization mechanism. Differences in hydrogen/deuterium exchange rates for macrocyclic and oxazalone structure peptide fragments are interpreted to be a result of the complex interplay of multiple basic sites in the peptide fragment, supporting the relay mechanism for deuterium exchange with CH(3)OD. Copyright (C) 2011 John Wiley & Sons, Ltd.

DOI10.1002/jms.1982
Division

GUTHz

Department

Moldyn

PID

4169f633a1f889943df135d2eb3c4f64

Alternate TitleJ. Mass Spectrom.

Go back one page.